<XML><RECORDS><RECORD><REFERENCE_TYPE>10</REFERENCE_TYPE><REFNUM>7072</REFNUM><AUTHORS><AUTHOR>Jones,A.</AUTHOR><AUTHOR>Wastling,J.</AUTHOR><AUTHOR>Hunt,E.</AUTHOR></AUTHORS><YEAR>2003</YEAR><TITLE>Proposal for two-dimensional gel electrophoresis data standards</TITLE><PLACE_PUBLISHED>DCS Tech Report</PLACE_PUBLISHED><PUBLISHER>N/A</PUBLISHER><PAGES>24</PAGES><ISBN>TR-2003-138</ISBN><LABEL>Jones:2003:7072</LABEL><KEYWORDS><KEYWORD>Proteomics; electrophoresis; standard;</KEYWORD></KEYWORDS<ABSTRACT>The global analysis of proteins is now feasible due to improvements in techniques such as two-dimensional gel electrophoresis (2-DE), mass spectrometry, yeast two-hybrid systems and the development of bioinformatics applications. The experiments form the basis of proteomics, and present significant challenges in data analysis, storage and querying. We have carried out case studies of research activities that use proteomics in microbial pathogenesis and cancer research. We argue that a standard format for proteome data is required to enable the storage, exchange and subsequent re-analysis of large data sets. We describe the criteria that must be met for the development of a standard for proteomics. We have developed a model to represent data from 2-DE experiments, including difference gel electrophoresis, along with image analysis and statistical analysis across multiple gels. This part of proteomics analysis is not represented in current proposals for proteomics standards. We are working with a standards organisation initiated at the European Bioinformatics Institute to develop a model encompassing biological sample origin, experimental protocols, a number of separation techniques and mass spectrometry. The standard format will facilitate the development of central repositories of data, enabling results to be verified or re-analysed, and the correlation of results produced by different research groups using a variety of laboratory techniques. </ABSTRACT></RECORD></RECORDS></XML>